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KMID : 0359320090490020121
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Korean Journal of Veterinary Research
2009 Volume.49 No. 2 p.121 ~ p.125
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Agreement of two ELISAs for Mycobacterium avium subspecies paratuberculosis in cattle in Korea
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Jung Byeong-Yeal
Lee O-Soo
Moon Oun-Kyoung
Lee Seong-Hyo
Kim Young-Hoan
Kim Ji-Yeon
Lee Su-Hwa
Hwang In-Yeong
Lee Kyung-Woo
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Abstract
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Paratuberculosis caused by Mycobacterium avium subspecies paratuberculosis (Mpt) is a chronic infectious enteric disease with deleterious impact on the performance in ruminants. In Korea, ELISA has been introduced to detect antibodies to Mpt in individual cattle. However, comparison study with ELISA has not been studied until now. In total, a panel of 899 serum samples obtained from dairy cattle was analyzed with two commercial ELISAs for Mpt to assess the performance. Two ELISAs employed in this study were both licensed worldwide. Two ELISAs applied onto same serum samples showed the moderate agreement (kappa value = 0.60). There was non-significant McNemar test (p = 0.0614) between two ELISA results indicating that each proportion detected by two kits did not differ. In addition, the percent agreement between two ELISA results was turned out to be 96.8% which interpreted excellent reproducibility. It was shown from this study that two ELISAs revealed moderate kappa agreement performance. The implication raised is that when ELISAs as diagnostics are used to detect Mpt in individual cattle, positive reaction by either ELISA should be interpreted as serologically Mpt positive due to presumed low sensitivity of ELISAs and their test agreement being less than 100%.
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KEYWORD
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agreement, dairy cattle, ELISA, kappa value, Mycobacterium avium subspecies paratuberculosis
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